Journal: mBio

Article Title: M2a macrophages facilitate resolution of chemically-induced colitis in TLR4-SNP mice

doi: 10.1128/mbio.01208-23

Figure Lengend Snippet: DSS-treated TLR4-SNP mice exhibit reduced M2a markers in the colon and reduced β-hydroxybutyrate (β-HB) levels in the sera. ( A ) M2a gene expression measured in the MC of male WT vs TLR4-SNP mice, comparing control, Day 9, and Day 11 samples by qRT-PCR. One-way ANOVA with Sidak multiple comparisons test. * P < 0.05; ** P < 0.01; **** P < 0.0001. Data are derived from two independent experiments. In total, control WT n = 11; control TLR4-SNP n = 15; WT Day 9 n = 13, Day 11 n = 13; TLR4-SNP Day 9 n = 15, Day 11 n = 15. ( B ) M2a (Ym1, Arg1) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data are representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. ( C ) M2a (Mrc1, PPARγ) protein production measured in DC samples in WT vs TLR4-SNP mice, comparing control and Day 11 samples. Data representative of results from two independent experiments totaling control WT n = 5; control TLR4-SNP n = 5; WT Day 11 n = 9; TLR4-SNP Day 11 n = 9. ( D ) β-HB concentration measured in the sera of control and DSS-treated, WT vs TLR4-SNP male mice at Days 9 and 11. Two-way ANOVA with Sidak multiple comparisons. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001. Data are derived from three independent experiments in which, together, control WT n = 12; control TLR4-SNP n = 13; WT Day 11 n = 10; TLR4-SNP Day 11 n = 10.

Article Snippet: Primary anti-PPARγ rabbit polyclonal antibody (Abclonal A0270; 1:2,000 dilution), primary mouse monoclonal anti-Arginase 1 (BD Biosciences 610709; 1:1,000 dilution), primary rabbit polyclonal anti-Ym1 (StemCell 60130; 1:1,000 dilution), primary goat polyclonal anti-Mrc1 (R&D Systems AF2535-SP; 1:1,000 dilution), primary rabbit anti-GAPDH (14C10) monoclonal antibody (Cell Signaling 2118; 1:1,000 dilution), secondary peroxidase-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch 111-035-003; 1:5,000 dilution), secondary peroxidase-conjugated goat anti-mouse IgG light chain-specific antibody (Jackson ImmunoResearch 115-035-174; 1:5,000 dilution), and secondary peroxidase-conjugated rabbit anti-goat IgG antibody (Jackson ImmunoResearch 305-035-003; 1:5,000 dilution) were used to detect species-specific primary antibodies.

Techniques: Expressing, Quantitative RT-PCR, Derivative Assay, Concentration Assay

Journal: mBio

Article Title: M2a macrophages facilitate resolution of chemically-induced colitis in TLR4-SNP mice

doi: 10.1128/mbio.01208-23

Figure Lengend Snippet: Administration of rosiglitazone, a PPARγ agonist ligand, ameliorates DSS-induced colonic damage in TLR4-SNP mice. Male TLR4-SNP mice were administered DSS starting on Day 0. On Days 2–7, mice were administered either saline or rosiglitazone (25 mg/kg) once daily i.p. ( A ) Average colonic symptom score over time in saline- and rosiglitazone-treated male TLR4-SNP mice. Two-way ANOVA with Sidak’s multiple comparisons; * P < 0.05. ( B ) Colon lengths at Day 14 in saline- and rosiglitazone-treated TLR4-SNP mice. Unpaired two-tailed Students t- test. *** P < 0.001. ( C ) Representative H&E-stained images of DC sections from saline- and rosiglitazone-treated TLR4-SNP mice at Day 14. ( D ) High-power image of submucosal infiltrating inflammatory cells in a representative DSS- and saline-treated TLR4-SNP mouse on Day 14. The infiltrating cells are predominantly mononuclear with occasional granulocytic cells (red arrows). ( E ) Right panel: histology scores of H&E-stained DC sections from saline- and rosiglitazone-treated TLR4-SNP mice at Day 14. Unpaired two-tailed Students t- test; ** P < 0.01. Results were derived from three independent experiments. In total, TLR4-SNP saline n = 17; TLR4-SNP rosiglitazone n = 19. ( F ) Ym1 (Chil3) protein expression at Day 11 in DC of DSS-treated TLR4-SNP mice therapeutically administered rosiglitazone or saline. Data are representative of results from two independent experiments totaling TLR4-SNP rosiglitazone-treated n = 9; TLR4-SNP saline-treated n = 8.

Article Snippet: Primary anti-PPARγ rabbit polyclonal antibody (Abclonal A0270; 1:2,000 dilution), primary mouse monoclonal anti-Arginase 1 (BD Biosciences 610709; 1:1,000 dilution), primary rabbit polyclonal anti-Ym1 (StemCell 60130; 1:1,000 dilution), primary goat polyclonal anti-Mrc1 (R&D Systems AF2535-SP; 1:1,000 dilution), primary rabbit anti-GAPDH (14C10) monoclonal antibody (Cell Signaling 2118; 1:1,000 dilution), secondary peroxidase-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch 111-035-003; 1:5,000 dilution), secondary peroxidase-conjugated goat anti-mouse IgG light chain-specific antibody (Jackson ImmunoResearch 115-035-174; 1:5,000 dilution), and secondary peroxidase-conjugated rabbit anti-goat IgG antibody (Jackson ImmunoResearch 305-035-003; 1:5,000 dilution) were used to detect species-specific primary antibodies.

Techniques: Saline, Two Tailed Test, Staining, Derivative Assay, Expressing

Journal: Frontiers in Pharmacology

Article Title: A Systematic Review of the Preventive and Therapeutic Effects of Naringin Against Human Malignancies

doi: 10.3389/fphar.2021.639840

Figure Lengend Snippet: Potential anticancer effects and related mechanisms of action of naringin based on in vitro studies.

Article Snippet: Esophageal , YM1 cell line , 300 μM , Sigma-Aldrich (St. Louis, MO, USA) , ND , Y , 24 h , ↓Cell proliferation, ↓cell viability , .

Techniques: In Vitro, Activity Assay, Inhibition, Transduction, Purification, Mass Spectrometry, Migration, High Performance Liquid Chromatography

Journal: Frontiers in Pharmacology

Article Title: A Systematic Review of the Preventive and Therapeutic Effects of Naringin Against Human Malignancies

doi: 10.3389/fphar.2021.639840

Figure Lengend Snippet: Potential anticancer effects and related mechanisms of action of naringin based on in vivo studies.

Article Snippet: Esophageal , YM1 cell line , 300 μM , Sigma-Aldrich (St. Louis, MO, USA) , ND , Y , 24 h , ↓Cell proliferation, ↓cell viability , .

Techniques: In Vivo, Animal Model, Purification, Liquid Chromatography

Journal: BMC Molecular and Cell Biology

Article Title: PYGO2 regulates IL10 and plays immunosuppressive role through ESCC progression

doi: 10.1186/s12860-025-00540-0

Figure Lengend Snippet: ( a ) The predicted protein-protein interaction network generated using GeneMANIA, illustrating the predicted relationships between PYGO2-IL10 through Wnt signaling pathway genes. ( b ) The protein-protein interaction network for PYGO2. ( c ) The protein-protein interaction network for IL10. ( d ) The predicted protein-protein interaction network in the context of ESCC, based on predictions from Phenolyzer

Article Snippet: KYSE-30 and YM1 ESCC lines were obtained from Pasteur Institute of Iran (Tehran, Iran), and cultured in RPMI-1640 and DMEM culture mediums (Bio-Idea, Iran) supplemented with 10% fetal bovine serum (FBS solution, BI, USA), and 100 μg/ml penicillin/streptomycin (Gipco, USA), and incubated in %90 humidity incubator containing %5 CO2 at 37 °C.

Techniques: Generated

Journal: BMC Molecular and Cell Biology

Article Title: PYGO2 regulates IL10 and plays immunosuppressive role through ESCC progression

doi: 10.1186/s12860-025-00540-0

Figure Lengend Snippet: Correlations between PYGO2 and IL10 gene expression and clinicopathological characteristics of ESCC patients

Article Snippet: KYSE-30 and YM1 ESCC lines were obtained from Pasteur Institute of Iran (Tehran, Iran), and cultured in RPMI-1640 and DMEM culture mediums (Bio-Idea, Iran) supplemented with 10% fetal bovine serum (FBS solution, BI, USA), and 100 μg/ml penicillin/streptomycin (Gipco, USA), and incubated in %90 humidity incubator containing %5 CO2 at 37 °C.

Techniques: Gene Expression

Journal: BMC Molecular and Cell Biology

Article Title: PYGO2 regulates IL10 and plays immunosuppressive role through ESCC progression

doi: 10.1186/s12860-025-00540-0

Figure Lengend Snippet: Descriptive analysis of PYGO2 and IL10 gene expression pattern in ESCC patients as scatter plot. The Y -axis represents the log 2 fold change of gene expression, and the X -axis indicates the number of patients. Relative mRNA expression of more than two-fold in tumor tissue is considered as overexpression; less than minus two-fold as underexpression and the range in between is defined as normal or no change

Article Snippet: KYSE-30 and YM1 ESCC lines were obtained from Pasteur Institute of Iran (Tehran, Iran), and cultured in RPMI-1640 and DMEM culture mediums (Bio-Idea, Iran) supplemented with 10% fetal bovine serum (FBS solution, BI, USA), and 100 μg/ml penicillin/streptomycin (Gipco, USA), and incubated in %90 humidity incubator containing %5 CO2 at 37 °C.

Techniques: Gene Expression, Expressing, Over Expression

Journal: BMC Molecular and Cell Biology

Article Title: PYGO2 regulates IL10 and plays immunosuppressive role through ESCC progression

doi: 10.1186/s12860-025-00540-0

Figure Lengend Snippet: Association between PYGO2 and IL10 expression in ESCC patients

Article Snippet: KYSE-30 and YM1 ESCC lines were obtained from Pasteur Institute of Iran (Tehran, Iran), and cultured in RPMI-1640 and DMEM culture mediums (Bio-Idea, Iran) supplemented with 10% fetal bovine serum (FBS solution, BI, USA), and 100 μg/ml penicillin/streptomycin (Gipco, USA), and incubated in %90 humidity incubator containing %5 CO2 at 37 °C.

Techniques: Expressing, Over Expression

Journal: BMC Molecular and Cell Biology

Article Title: PYGO2 regulates IL10 and plays immunosuppressive role through ESCC progression

doi: 10.1186/s12860-025-00540-0

Figure Lengend Snippet: Potential correlation between PYGO2 and IL10 expression through the Wnt/β-catenin signaling pathway in ESCC progression. Binding of Wnt ligand to FZD and LRP induces inactivation of the AXIN - APC - GSK3β destruction complex via DVL , leading to β-catenin accumulation and transition to the nucleus. Then, PYGO2 as a coactivator of the transcriptional complex ( LEF / TCF / β-catenin ), may directly activate transcription of target genes such as IL10 . Furthermore, IL10 expression manifests direct correlation with FZD, WNT, LRP expression, β-catenin accumulation, and augmentation of EGFR levels in this pathway

Article Snippet: KYSE-30 and YM1 ESCC lines were obtained from Pasteur Institute of Iran (Tehran, Iran), and cultured in RPMI-1640 and DMEM culture mediums (Bio-Idea, Iran) supplemented with 10% fetal bovine serum (FBS solution, BI, USA), and 100 μg/ml penicillin/streptomycin (Gipco, USA), and incubated in %90 humidity incubator containing %5 CO2 at 37 °C.

Techniques: Expressing, Binding Assay

Journal: Frontiers in Immunology

Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

doi: 10.3389/fimmu.2014.00430

Figure Lengend Snippet: Western blot analysis of the expressions of collagen type I, IRF-5 (M1), IL-12 (M1), and YM-1 (M2) in normal and fibrotic mouse (A) and human (B) livers . * p < 0.05. N = 5 cirrhotic human livers, N = 6 normal human livers, N = 4 normal mouse livers, N = 6 fibrotic mouse livers.

Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

Techniques: Western Blot

Journal: Frontiers in Immunology

Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

doi: 10.3389/fimmu.2014.00430

Figure Lengend Snippet: Localization TGM-2 (M2) in human livers . (A,B) Immunohistochemical localization of TGM-2 in normal (A) and cirrhotic (B) human livers. Note the presence of the strongly stained cells in the fibrotic matrix (F) . (C) Co-localization of TGM-2 (blue staining) and CD68 (red staining). (D) Co-localization of TGM-2 (blue staining) and CD206 (red staining). Arrows indicate co-localization. Magnifications: 100× (A,B) and 400× (C,D) . N = 5 cirrhotic human livers, N = 6 normal human livers.

Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

Techniques: Immunohistochemical staining, Staining

Journal: Frontiers in Immunology

Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

doi: 10.3389/fimmu.2014.00430

Figure Lengend Snippet: Localization of YM-1 (M2) in mouse livers . (A) Co-localization of YM-1 (red staining) and CD68 (blue staining). (B) Co-localization of YM-1 (red staining) and CD206 (blue staining). (C–F) Immunohistochemical localization of YM-1 in livers of normal mice (C,E) and in advanced fibrosis (D,F) . Magnifications: 40× (C,D) , 200× (E,F) , and 400× (A,B) . N = 4 normal mouse livers, N = 6 fibrotic mouse livers.

Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

Techniques: Staining, Immunohistochemical staining

Journal: Frontiers in Immunology

Article Title: Hepatic Localization of Macrophage Phenotypes during Fibrogenesis and Resolution of Fibrosis in Mice and Humans

doi: 10.3389/fimmu.2014.00430

Figure Lengend Snippet: Expressions of YM-1 (M2) in fibrotic mouse livers [4 weeks CCl4 (A,B)] and in fibrotic livers undergoing resolution [after cessation of CCl4 administration (C,D)] . Immunohistochemical pictures demonstrate an overview [ (A,C) magnification 40×] and close up [ (B,D) magnification 200×]. (E) Western blot quantification of YM-1 expression in fibrosis versus resolution. ** p < 0.01. N = 6/group.

Article Snippet: Sections were incubated with the primary antibody for 1 h. Primary antibodies to detect fibrotic extracellular matrix (polyclonal goat anti-collagen type I from Southern Biotech), macrophages [mouse anti-human CD68 (DAKO), monoclonal rat anti-mouse CD68 (AbD Serotec, Düsseldorf, Germany), and polyclonal rabbit anti-human CD68 (Santa Cruz Biotechnology)], M1 macrophages [polyclonal rabbit anti-human and mouse IRF-5 (Protein Tech, Manchester, UK), goat polyclonal anti-human IL-12 p40 antibody (ThermoScientific), and goat polyclonal anti-human and mouse MMP9 (Santa Cruz)], and M2 macrophages [polyclonal goat anti-mouse chitinase 3-like/ECF-L (YM-1; R&D), rabbit anti-human TGM-2 (AbD Serotec) and CD206 (rat anti-mouse CD206 and mouse anti-human CD206) both from BioLegends (ITK Diagnostics, Uithoorn, The Netherlands)] were used.

Techniques: Immunohistochemical staining, Western Blot, Expressing